“Massive and strikingly similar changes of the nuclear landscape during preimplantation development of fertilized and cloned bovine embryos”. Thomas Cremer

Thomas Cremer was one of the first supporter of the idea that high order chromatin arrangement and architecture of the nucleus are essential for fundamental nuclear functions. T.Cremer work is focused on the comparison of nuclear phenotypes in a variety of developmental stages in order to associate the nuclear architecture with specific features of the cells using three-dimensional confocal analysis and structures illumination microscopy (3D-SIM) techniques. As a pointed paint, where a cloud of pixels composes a unique final image, the analysis of the chromatin architecture within the nucleus at high resolution gives an image of the chromatin organization that control the cellular identity regulating the gene expression profile. Thanks to T.Cremer’s photographies at small resolution of the nuclear compartment of cloned embryos obtained from the transfer of a somatic nucleus in the bovine oocytes, important similarities and differences among different developmental stages were suggested.